Observing one-divalent-metal-ion-dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo
Metal-ion-dependent nucleases play crucial roles in cellular defense and biotechnological applications.Time-resolved crystallography has resolved catalytic details of metal-ion-dependent DNA hydrolysis and synthesis, uncovering the essential roles of multiple metal ions during catalysis.The histidine-metal (His-Me) superfamily nucleases are renowne